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Sains Malaysiana 53(3)(2024): 575-589
http://doi.org/10.17576/jsm-2024-5303-08
Proteomics Shows the Role of Paederia scandens in
Ameliorating Non-alcoholic Fatty Liver Disease in a Rat Model
(Proteomik Menunjukkan Peranan Paederia scandens dalam memulihkan Penyakit Hati Berlemak Bukan Alkohol pada Model Tikus)
JING WANG1†, TIEJIN TONG1†& QIANG WU1,*
Agricultural College, Yibin Vocational and
Technical College, Yibin 644000, China
Received: 6 July
2023/Accepted: 9 February 2024
Abstract
This study was conducted to evaluate the effect of Paederia scandens on high-fat dietinduced non-alcoholic fatty liver disease (NAFLD) in a rat model and further show the
therapeutic mechanisms of Paederia scandens. Thirty rats weighing 180 ± 12 g (6 weeks old) were randomly divided into three
groups: A control group (CG), a high-fat diet model group (HF), and a Paederia scandens intervention group (PS). After 45 days, the
rats' serum lipid metabolism, liver injury parameters, and liver proteomics
were detected. The results indicated that dietary Paederia scandens significantly reduced the
levels of triglycerides, total cholesterol, glucose, and low-density
lipoprotein cholesterol in the NAFLD rats compared with those in the HF group.
Meanwhile, decreased levels of alanine aminotransferase and aspartate
transaminase were observed in rats of the PS group. In addition, 382
differentially abundant proteins were identified between the HF and PS groups.
Protein-protein interaction network analysis identified 14 keystone proteins
that might play critical roles in ameliorating NAFLD. In particular, Paederia scandens treatment significantly upregulated the levels of Hadh, Hadhb, Acadl, Acox1, Acox3,
Cyp3a2, and Cyp1a1, which are involved in fatty acid β‑oxidation,
PPAR, and cytochrome P450 signaling pathways. Hence, the data demonstrated that Paederia scandens ameliorates hepatic lipid accumulation
and impairment by enhancing fatty acid β-oxidation and activating PPAR and
cytochrome P450 signaling pathways. These data provide new insights into the
treatment of NAFLD and suggest the potential of Paederia scandens as an effective therapy.
Keywords: Non-alcoholic fatty liver
disease; Paederia scandens; proteomics; rat; therapeutic
mechanisms
Abstrak
Penyelidikan ini dijalankan untuk menilai kesan Paederia scandens terhadap penyakit hati berlemak bukan alkohol (NAFLD) akibat diet tinggi lemak pada model tikus dan seterusnya menunjukkan mekanisme terapeutik Paederia scandens. Tiga puluh ekor tikus dengan berat 180 ± 12 g (6 minggu) dibahagikan secara rawak kepada tiga kumpulan: Kumpulan kawalan (CG), kumpulan model diet tinggi lemak (HF) dan kumpulan intervensi Paederia scandens (PS). Selepas 45 hari, metabolisme lipid serum tikus,
parameter kecederaan hati dan proteomik hati telah dikesan. Keputusan menunjukkan bahawa diet Paederia scandens secara signifikan mengurangkan tahap trigliserida, jumlah kolesterol, glukosa dan kolesterol lipoprotein berketumpatan rendah pada tikus NAFLD berbanding dengan kumpulan HF. Sementara itu, penurunan tahap alanine aminotransferase dan aspartate transaminase diperhatikan pada tikus kumpulan PS. Di samping itu, 382
protein yang banyak berbeza telah dikenal pasti antara kumpulan HF dan PS. Analisis rangkaian interaksi protein-protein mengenal pasti 14 protein kiston yang mungkin memainkan peranan penting dalam memperbaiki NAFLD. Khususnya, rawatan Paederia scandens dengan ketara mengimbangi tahap Hadh, Hadhb, Acadl, Acox1, Acox3,
Cyp3a2 dan Cyp1a1 yang terlibat dalam pengoksidaan β asid lemak, PPAR dan laluan isyarat sitokrom P450. Oleh itu, data menunjukkan bahawa Paederia scandens memperbaiki pengumpulan dan kemerosotan lipid hepatik dengan meningkatkan pengoksidaan β asid lemak dan mengaktifkan laluan isyarat PPAR dan sitokrom P450. Data ini memberikan pandangan baharu tentang rawatan NAFLD dan mencadangkan potensi Paederia scandens sebagai terapi yang berkesan.
Kata kunci: Mekanisme terapeutik; penyakit hati berlemak bukan alkohol; Paederia scandens; proteomik; tikus
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*Corresponding
author; email: wuqiangfirst@163.com
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